Composite

Part:BBa_I716321:Design

Designed by: Hannah Cole   Group: iGEM07_Berkeley_UC   (2007-08-08)


Myoglobin promoter rbs terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal EcoRI site found at 2696
    Illegal XbaI site found at 367
    Illegal XbaI site found at 420
    Illegal PstI site found at 640
    Illegal PstI site found at 2769
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal EcoRI site found at 2696
    Illegal PstI site found at 640
    Illegal PstI site found at 2769
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 625
    Illegal EcoRI site found at 331
    Illegal EcoRI site found at 2696
    Illegal BglII site found at 2705
    Illegal BamHI site found at 2754
    Illegal XhoI site found at 2763
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal EcoRI site found at 2696
    Illegal XbaI site found at 367
    Illegal XbaI site found at 420
    Illegal PstI site found at 640
    Illegal PstI site found at 2769
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 331
    Illegal EcoRI site found at 2696
    Illegal XbaI site found at 367
    Illegal XbaI site found at 420
    Illegal PstI site found at 640
    Illegal PstI site found at 2769
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1727
    Illegal BsaI.rc site found at 3850


Design Notes

3 Internal restriction sites in original gene.


Source

Sperm whale myoglobin

References